17:00–17:15 | ABSTRACT 1339
ASSESSING THE EFFECTS OF TEMPERATURE AND SALINITY ON DINOFLAGELLATE BIOLUMINESCENCE
Mechanically stimulated bioluminescence can be broken down into first flash kinetic parameters (FFKPs), which has potential for in situ identification of planktonic species (Letendre et al. 2024). However, the impact of variable environmental factors on FFKPs has yet to be rigorously tested for many species. Multiple growth chambers with 6 temperatures ranging from 10°C to 30°C and media with 5 salinities from 34 to 42 ppt housed a minimum of 4 generations from Pyrocystis fusiformis, Pyrocystis noctiluca, Pyrodinium bahamense, Lingulodinium polyedra, and Alexandrium monilatum. These temperatures and salinities provided 30 independent environments for which bioluminescence emissions were tested in Lab-CODEX, a first flash bioluminescent measurement device with high-flow inducing mechanical stimulation characterized with CFD modeling and developed at Harbor Branch Oceanographic Institute (HBOI). Individual FFKPs, i.e., rise time, decay time, flash duration, peak intensity, E-fold time and integrated emissions, were extracted from each flash. Measured intraspecies FFKPs were compared from the different growing environments. Temperature and salinity interactions strongly influenced FFKPs in P. fusiformis. However, salinity alone exhibited zero slopes, indicating a minimal effect on FFKP variability under constant temperature conditions, particularly at 23°C. In contrast, temperature alone had a significant effect on FFKPs. Overall, the interaction of these two parameters is strongly significant, highlighting temperature as the dominant driver, with salinity modulating the magnitude and structure of responses across temperature treatments. Results from these tests could help in detecting these species in the field growing within their full homeostatic range.
Abigail Blackburn, Harbor Branch Oceanographic Institute, [email protected], https://orcid.org/0000-0002-9903-3181
Francis Letendre, HBOI FAU, [email protected]
Owen Dillahay, HBOI FAU, [email protected]
Michael Twardowski, HBOI FAU, [email protected]
